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Copepod.Com |

| Species | Nitokra lacustris (Harpacticoid) | |
| Motility | Planktonic (in the water column) | |
| Size | 40 - 270 microns | |
| Application |
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| Life Cycle | Eggs -> Nauplii -> Copepodites (Juveniles) -> Adults - Females bearing egg sacs |
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| Reproduction | The females only need to mate once in order to have 3 to5 sacs of eggs with 35 eggs each. | |
| How long can a culture be maintained? | Indefinitely if treated correctly | |
| What is the doubling time? | 2-3 days, depending upon temperature and feed |
In batch cultures use very low aeration. Treat water with chlorine or filters (to 1 micron) before using. The water should be at 30 ppt salinity and 24 to 28 degrees Celsius for optimum growth. Add copepods, and 100,000 to 200,000 cells algae concentrate (recommend Thalasiosira, Pavlova, Isochrysis or Tetraselmis). Add more algae every 9 to 10 days, depending on level of productivity. It is important to be conservative with the feed.
Periodically (every one to two months) filter and restart the cultures to remove detritus. Filter for small juveniles with 35 micron sieve and adults with 125 micron sieve. Use smaller stages for fish feed, retrieve females with egg sacs for restarting cultures.
Restart cultures with females bearing egg sacs. (About 10/L if you have low numbers, 50 to 100/L will result in faster population growth.)
This formula works for every size container, used in batch culture (meaning no recirculation). It may be possible to increase population growth by removing ciliates by filtration - this is not known yet.
These copepods are about 50% larger than a rotifer which means that they will be hard to see with the naked eye.
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