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Receiving your Rotifers and setting up your Rotifer Culture

Once you receive your shipment of rotifers use the following protocols to make sure you get the best survival rates:

1. Open the box and store the bags of rotifers in a refrigerator (4-8 C) until you are ready to put them in the rotifer tank.
2. Measure the pH in the bag of rotifers (it will probably be about 7) and adjust the pH in your culturing tank to be the same. Otherwise the rotifers can be pH "shocked" and many of them can die. The pH will naturally return to its normal values within several hours and the rotifers will adjust without any problems. For information about adjusting pH click here.
3. The rotifers will arrive at a salinity of roughly 20 ppt (1.015).  Your water does NOT need to be adjusted to this salinity - they will go into water from 20 to 30 ppt (1.023) without any problems.
4. Add the recommend amount of ClorAm-X or Ultimate to the tank to buffer the water. Dosage rates can be found at www.cloram-x.com.
5. Take the bag of rotifers from the refrigerator and put it in the rotifer tank for 10-15 minutes to allow it to slowly warm up.  Then cut the bag open and release the rotifers into the tank.
6. Add enough Nannochloropsis or "Rotifer Diet" to the water to establish a green background.
7. Check the rotifer tank in 2 hours to see if more algae is needed. The rotifers will be hungry after their trip and will consume more than usual amount for the first few hours.

For information about the life span of the rotifers during transit and refrigeration click here.

For more information please visit our website at www.Rotifer.com.

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